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whole genome (wg) multilocus sequence typing (mlst)  (Ridom GmbH)

 
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    Ridom GmbH whole genome (wg) multilocus sequence typing (mlst)
    Whole Genome (Wg) Multilocus Sequence Typing (Mlst), supplied by Ridom GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multilocus+sequence+type+(mlst)/core+genome+multilocus+sequence+typing++cgmlst++scheme/pm39708273-5-12-20
    Average 90 stars, based on 1 article reviews
    whole genome (wg) multilocus sequence typing (mlst) - by Bioz Stars, 2026-09
    90/100 stars

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    The information on primers used for 7 MLST housekeeping genes.

    Journal: Poultry Science

    Article Title: High-throughput Oxford Nanopore sequencing-based approach for the multilocus sequence typing analysis of large-scale avian Escherichia coli study in Mississippi

    doi: 10.1016/j.psj.2024.104067

    Figure Lengend Snippet: The information on primers used for 7 MLST housekeeping genes.

    Article Snippet: Workflow of multilocus sequence typing (MLST) using Oxford Nanopore Technologies (ONT).

    Techniques: Sequencing, Amplification

    Optimized multiplex PCR reactions targeting for 7 housekeeping gene alleles used in E. coli MLST analysis.

    Journal: Poultry Science

    Article Title: High-throughput Oxford Nanopore sequencing-based approach for the multilocus sequence typing analysis of large-scale avian Escherichia coli study in Mississippi

    doi: 10.1016/j.psj.2024.104067

    Figure Lengend Snippet: Optimized multiplex PCR reactions targeting for 7 housekeeping gene alleles used in E. coli MLST analysis.

    Article Snippet: Workflow of multilocus sequence typing (MLST) using Oxford Nanopore Technologies (ONT).

    Techniques: Multiplex Assay

    Workflow of multilocus sequence typing (MLST) using Oxford Nanopore Technologies (ONT). A general workflow for the DNA extraction, amplicon generation, amplicon barcoding, ONT sequencing, and data analysis.

    Journal: Poultry Science

    Article Title: High-throughput Oxford Nanopore sequencing-based approach for the multilocus sequence typing analysis of large-scale avian Escherichia coli study in Mississippi

    doi: 10.1016/j.psj.2024.104067

    Figure Lengend Snippet: Workflow of multilocus sequence typing (MLST) using Oxford Nanopore Technologies (ONT). A general workflow for the DNA extraction, amplicon generation, amplicon barcoding, ONT sequencing, and data analysis.

    Article Snippet: Workflow of multilocus sequence typing (MLST) using Oxford Nanopore Technologies (ONT).

    Techniques: Sequencing, DNA Extraction, Amplification

    Summary of the ONT sequencing results from ONT-MLST Beta workflow.

    Journal: Poultry Science

    Article Title: High-throughput Oxford Nanopore sequencing-based approach for the multilocus sequence typing analysis of large-scale avian Escherichia coli study in Mississippi

    doi: 10.1016/j.psj.2024.104067

    Figure Lengend Snippet: Summary of the ONT sequencing results from ONT-MLST Beta workflow.

    Article Snippet: Workflow of multilocus sequence typing (MLST) using Oxford Nanopore Technologies (ONT).

    Techniques: Sequencing, Generated

    Simulation of accuracy of the allele and ST calls for each subsample of the ONT compared to the SRST2 (Illumina) output. The sequencing data of 66 E. coli isolates from ONT-MLST Beta workflow and Illumina platform was used in this in silico simulation analysis.

    Journal: Poultry Science

    Article Title: High-throughput Oxford Nanopore sequencing-based approach for the multilocus sequence typing analysis of large-scale avian Escherichia coli study in Mississippi

    doi: 10.1016/j.psj.2024.104067

    Figure Lengend Snippet: Simulation of accuracy of the allele and ST calls for each subsample of the ONT compared to the SRST2 (Illumina) output. The sequencing data of 66 E. coli isolates from ONT-MLST Beta workflow and Illumina platform was used in this in silico simulation analysis.

    Article Snippet: Workflow of multilocus sequence typing (MLST) using Oxford Nanopore Technologies (ONT).

    Techniques: Sequencing, In Silico